Stoichiometric inconsistency violates universal constraints: 1. Molecular masses are always positive, and 2. On each side of a reaction the mass is conserved. A single incorrectly defined reaction can lead to stoichiometric inconsistency in the model, and consequently to unconserved metabolites. Similar to insufficient constraints, this may give rise to cycles which either produce mass from nothing or consume mass from the model. Implementation: This test first uses an implementation of the algorithm presented in section 3.1 by Gevorgyan, A., M. G Poolman, and D. A Fell. "Detection of Stoichiometric Inconsistencies in Biomolecular Models." Bioinformatics 24, no. 19 (2008): 2245. doi: 10.1093/bioinformatics/btn425 Should the model be inconsistent, then the list of unconserved metabolites is computed using the algorithm described in section 3.2 of the same publication.

This model contains 0 (0.00%) unconserved metabolites: